NAPB Polyclonal antibody, PBS Only

NAPB Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14106-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 14106-1-AP (same clone as 14106-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14106-1-AP (NAPB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 14106-1-AP (same clone as 14106-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14106-1-AP (NAPB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 14106-1-AP (same clone as 14106-1-PBS)

  IP result of anti-NAPB (IP:14106-1-AP, 4ug; Detection:14106-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 14106-1-AP (same clone as 14106-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14106-1-AP (NAPB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 14106-1-AP (same clone as 14106-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14106-1-AP (NAPB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 14106-1-AP (same clone as 14106-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14106-1-AP (NAPB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14106-1-AP (same clone as 14106-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using NAPB antibody 14106-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

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Product Information

14106-1-PBS targets NAPB in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NAPB fusion protein Ag5239
• Full Name: N-ethylmaleimide-sensitive factor attachment protein, beta
• Calculated Molecular Weight: 298 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC060840
• Gene Symbol: NAPB
• Gene ID (NCBI): 63908
• RRID: AB_2185010
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.