NANOS3 Polyclonal antibody

NANOS3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 21679-1-AP

Synonyms

Nanos homolog 3, nanos homolog 3 (Drosophila), NANOS1L, NANOS3, NOS 3, NOS3

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Validation Data Gallery

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  WB analysis of mouse testis using 21679-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21679-1-AP (NANOS3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 21679-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 21679-1-AP (NANOS3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 21679-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 21679-1-AP (NANOS3 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

The immunogen of 21679-1-AP is NANOS3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NANOS3 fusion protein Ag16014
• Full Name: nanos homolog 3 (Drosophila)
• Calculated Molecular Weight: 192 aa, 21 kDa
• Observed Molecular Weight: 26-30 kDa
• GenBank Accession Number: BC101209
• Gene Symbol: NANOS3
• Gene ID (NCBI): 342977
• RRID: AB_10859250
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The NANOS gene family is required for germ cell development in diverse organisms. NANOS3 is found in migrating primordial germ cells, and the homozygous deficiency of NANOS3 in the mouse results in the complete loss of germ cells in both sexes.

Publications

Species	Application	Title
mouse	IF	Nucleic Acids Res; Regulation of both transcription and RNA turnover contribute to germline specification.; Authors - Kun Tan
mouse,rat	WB,IF	Environ Pollut; Wnt10a downregulation contributes to MEHP-induced disruption of self-renewal and differentiation balance and proliferation inhibition in GC-1 cells: Insights from multiple transcriptomic profiling; Authors - Yifan Hong
human	WB,IHC,IF	J Pathol; The human NANOS3 gene contributes to lung tumour invasion by inducing epithelial-mesenchymal transition.; Authors - Simon Grelet
rat	WB	Exp Cell Res; Pum2 mediates Sirt1 mRNA decay and exacerbates hypoxia/reoxygenation-induced cardiomyocyte apoptosis.; Authors - Yuanping Cao
human	WB,IHC	BMC Cancer; A new mouse model to study the role of ectopic Nanos3 expression in cancer.; Authors - Vanessa Andries
mouse	WB	J Ethnopharmacol; Zhenwu decoction ameliorates cardiac hypertrophy through activating sGC (soluble guanylate cyclase) - cGMP (cyclic guanosine monophosphate) - PKG (protein kinase G) pathway; Authors - Liqian Chen