NAGK Polyclonal antibody

• Featured Product
• KD/KO Validated

NAGK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15051-1-AP

Synonyms

HSA242910, GNK, GlcNAc kinase, EC:2.7.1.60, EC:2.7.1.59

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Validation Data Gallery

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  WB analysis using 15051-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15051-1-AP (NAGK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 15051-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15051-1-AP (NAGK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 15051-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15051-1-AP (NAGK antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 15051-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15051-1-AP (NAGK antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 15051-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NAGK antibody (15051-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: HEK-293T cells, mouse testis tissue, HeLa cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

15051-1-AP targets NAGK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NAGK fusion protein Ag7034
• Full Name: N-acetylglucosamine kinase
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC001029
• Gene Symbol: NAGK
• Gene ID (NCBI): 55577
• RRID: AB_2152368
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Elife; Glutamine deprivation triggers NAGK-dependent hexosamine salvage.; Authors - Sydney Campbell ; KD Validated