NAF1 Polyclonal antibody

NAF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27764-1-AP

Synonyms

hNAF1, H/ACA ribonucleoprotein complex non-core subunit NAF1

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Validation Data Gallery

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  WB analysis of K-562 using 27764-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 27764-1-AP (NAF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 27764-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 27764-1-AP (NAF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 27764-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27764-1-AP (NAF1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 27764-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27764-1-AP (NAF1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 27764-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27764-1-AP (NAF1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells, HL-60 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27764-1-AP targets NAF1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NAF1 fusion protein Ag26787
• Full Name: nuclear assembly factor 1 homolog (S. cerevisiae)
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC008207
• Gene Symbol: NAF1
• Gene ID (NCBI): 92345
• RRID: AB_2880965
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NAF1, an H/ACA box RNP assembly chaperone, is a factor necessary for H/ACA snoRNP maturation and ribosome biosynthesis in mammalian cells. In addition, among these RNA biogenesis factors, NAF1 may be unique in that it is required at full dosage for telomerase and telomere maintenance in the process of pulmonary fibrosis-emphysema. (PMID: 30936423)