APPBP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

APPBP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14863-1-AP

Synonyms

NAE1, HPP1, HPP 1, APP-BP1, APP BP1

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Validation Data Gallery

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  WB analysis of human kidney using 14863-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14863-1-AP (APPBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14863-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14863-1-AP (APPBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14863-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14863-1-AP (APPBP1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 14863-1-AP

  IP result of anti-APPBP1 (IP:14863-1-AP, 4ug; Detection:14863-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of mouse salivary gland using 14863-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse salivary gland tissue slide using 14863-1-AP (APPBP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human kidney tissue, human brain tissue, mouse brain tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: mouse salivary gland tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

14863-1-AP targets APPBP1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APPBP1 fusion protein Ag6672
• Full Name: NEDD8 activating enzyme E1 subunit 1
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC000480
• Gene Symbol: NAE1/APPBP1
• Gene ID (NCBI): 8883
• RRID: AB_2251388
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
	WB	Proc Natl Acad Sci U S A; Polo-like kinases mediate cell survival in mitochondrial dysfunction.; Authors - Takumi Matsumoto ; KD Validated
human	IHC	Heliyon; Dual role of targeting NAE1 in nasopharyngeal carcinoma: Antitumor effects yet inducing radiotherapy resistance; Authors - Qinsong Liu