NADK Recombinant antibody, PBS Only (Capture)

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  WB analysis using 84996-1-RR (same clone as 84996-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84996-1-RR (NADK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84996-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01732-1

  Cytometric bead array standard curve of MP01732-1, NADK Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84996-1-PBS. Detection antibody: 84996-2-PBS. Standard: Ag7906. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84996-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84996-1-RR against Human NADK were performed. The affinity constant is 2.25 nM.

NADK {ptg:Brand} Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 84996-1-PBS

Clone No.242502B7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

Poly(P)/ATP NAD kinase, NAD kinase, EC:2.7.1.23, 242502B7

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Product Information

84996-1-PBS targets NADK as part of a matched antibody pair:

MP01732-1: 84996-1-PBS capture and 84996-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NADK fusion protein Ag7906
• Full Name: NAD kinase
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 49-52 kDa
• GenBank Accession Number: BC001709
• Gene Symbol: NADK
• Gene ID (NCBI): 65220
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95544
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

NAD kinase (NADK) is the sole NADP+-biosynthetic enzyme that catalyzes phosphorylation of NAD+ to yield NADP+ using ATP as a phosphoryl donor, and thus, plays a vital role in the cell and represents a potentially powerful antimicrobial drug target (PMID:21526340). It belongs to the NAD kinase family. NADK has 3 isoforms with the molecular mass of 49, 63 and 46 kDa. The catalytically active human NADK is a homotetramer (PMID:11594753).