NAAA Polyclonal antibody

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  WB analysis using 16576-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16576-1-AP (NAAA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

NAAA Polyclonal Antibody for WB, ELISA

Cat No. 16576-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ASAH-like protein, ASAHL, ASAH like protein, Acylsphingosine deacylase NAAA, Acid ceramidase-like protein

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16576-1-AP targets NAAA in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NAAA fusion protein Ag9861
• Full Name: N-acylethanolamine acid amidase
• Calculated Molecular Weight: 359 aa, 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC011854
• Gene Symbol: NAAA
• Gene ID (NCBI): 27163
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q02083
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NAAA (N-acylethanolamine acid amidase), also named ASAHL and PLT, is a novel lysosomal hydrolase structurally and functionally similar to acid ceramidase and is mainly involved in the hydrolysis of fatty amides. NAAA is mainly responsible for the degradation of bioactive fatty amides, such as palmitoylethanolamide (PEA), and is involved in lipid metabolism and fatty acid metabolism processes (PMID: 32191459; PMID: 17712833).