N4BP3 Polyclonal antibody

• Featured Product
• KD/KO Validated

N4BP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 16733-1-AP

Synonyms

NEDD4-binding protein 3, Nedd4 binding protein 3, KIAA0341

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Validation Data Gallery

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  WB analysis using 16733-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16733-1-AP (N4BP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16733-1-AP

  IP result of anti-N4BP3 (IP:16733-1-AP, 4ug; Detection:16733-1-AP 1:2000) with mouse brain tissue lysate 1800 ug.

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  IHC staining of mouse kidney using 16733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16733-1-AP (N4BP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 16733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16733-1-AP (N4BP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16733-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16733-1-AP (N4BP3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 16733-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16733-1-AP (N4BP3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 16733-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16733-1-AP (N4BP3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 16733-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16733-1-AP (N4BP3 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HEK-293 using 16733-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using N4BP3 antibody (16733-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HEK-293 using 16733-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using N4BP3 antibody (16733-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse kidney tissue, human cervical cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IP: See 1 publications below

Product Information

16733-1-AP targets N4BP3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: N4BP3 fusion protein Ag10194
• Full Name: Nedd4 binding protein 3
• Calculated Molecular Weight: 544 aa, 60 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC053323
• Gene Symbol: N4BP3
• Gene ID (NCBI): 23138
• RRID: AB_2878308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

N4BP3, also named as KIAA0341, a Fezzin family member, during nerve cell development. It is crucial for axonal and dendritic branching in developing neurons. It binds the protein NEDD4 and interacts with 14-3-3 proteins. N4BP3 is present in early neurites, growth cones, axons and dendrites of developing primary hippocampal neurons.

Publications

Species	Application	Title
human	WB,IP	Front Microbiol; N4BP3 Regulates RIG-I-Like Receptor Antiviral Signaling Positively by Targeting Mitochondrial Antiviral Signaling Protein.; Authors - Chen Wang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wojciech (Verified Customer) (05-25-2023)

I used this antibody to assess protein levels in human T-ALL cells by western blot. I got 2 unspecific bands (40 and ~200 kDa). Signal very strong. Dilution: 1:1000 with 50 ug of lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: T-ALL cell line