N4BP2 Polyclonal antibody

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  WB analysis using 23892-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23892-1-AP (N4BP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

N4BP2 Polyclonal Antibody for WB, ELISA

Cat No. 23892-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

NEDD4 binding protein 2, KIAA1413, B3BP

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23892-1-AP targets N4BP2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: N4BP2 fusion protein Ag20943
• Full Name: NEDD4 binding protein 2
• Calculated Molecular Weight: 1770 aa, 199 kDa
• Observed Molecular Weight: 199 kDa
• GenBank Accession Number: BC126466
• Gene Symbol: N4BP2
• Gene ID (NCBI): 55728
• RRID: AB_3669422
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q86UW6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

N4BP2 (Nedd4 binding protein 2) is a Bcl-3 binding protein, which contains a polynucleotide kinase domain (PNK) at the N-terminus and a Small MutS Related (Smr) domain with nicking endonuclease activity near C-terminus. N4BP2 was at higher levels in a majority of tumor tissues examined, relative to paired normal tissues (PMID: 17626640).