Myogenin Polyclonal antibody

• 

  WB analysis using 26762-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26762-1-AP (Myogenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Myogenin Polyclonal Antibody for WB, ELISA

Cat No. 26762-1-AP

Publications(1)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

MYOG, Myf-4, MYF4, Myf 4, Class C basic helix-loop-helix protein 3

---

Tested Applications

• Positive WB detected in: mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

26762-1-AP targets Myogenin in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Myogenin fusion protein Ag25081
• Full Name: myogenin (myogenic factor 4)
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC053899
• Gene Symbol: Myogenin
• Gene ID (NCBI): 4656
• RRID: AB_3669566
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P15173
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYOG (encoded Myogenin protein) is a muscle-specific transcription factor that belongs to a family of basic helix-loop-helix transcription factors. It plays a role in muscle differentiation, cell cycle exit, and muscle atrophy(PMID: 30315160). Diseases associated with MYOG include Embryonal Rhabdomyosarcoma and Gallbladder Sarcoma.

Publications

Species	Application	Title
rat	WB	Int Urogynecol J; Mechanical Stress-Oxidative Stress Axis: Biological Basis in the Vaginal Wall and Pelvic Floor Muscles of Rats with Simulated Birth Injury; Authors - Qing Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Kazuaki (Verified Customer) (01-28-2025)

C2C12 cells differentiated for 5 days by using DMEM with 2%HS and 1%PS

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse myoblast C2C12 cell line

Myogenin Antibody Western Blot validation (1:1000 dilution) in Mouse myoblast C2C12 cell line (Cat no:26762-1-AP)