Mouse/Rat FGF-21 ELISA Kit
Cat no : KE10095
Synonyms
Fgf21, fibroblast growth factor 21
Validation Data Gallery
Product Information
KE10095 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse/Rat FGF-21 ELISA kit is to be used to detect and quantify protein levels of endogenous FGF-21. The assay recognizes mouse/rat FGF-21. An antibody specific for FGF-21 has been pre-coated onto the microwells. The FGF-21 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for FGF-21 is added to detect the captured FGF-21 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse/Rat FGF-21 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 19.3 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 56636 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 110% | 91%-120% |
| Rat serum | 91% | 83%-100% |
| Cell culture supernatant | 110% | 98%-120% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,033.8 | 34.9 | 3.4 |
| 2 | 20 | 258.7 | 11.2 | 4.3 |
| 3 | 20 | 62.5 | 4.4 | 7.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,040.3 | 61.0 | 5.9 |
| 2 | 24 | 250.3 | 18.5 | 7.4 |
| 3 | 24 | 125.3 | 10.4 | 8.3 |
Background Information
Fibroblast growth factor 21 (FGF21) is a peptide hormone that is expressed across several tissues, predominantly in liver and adipocyte tissues, and, to a lower extent, in the pancreas, skeletal muscle, heart, kidneys and testes. FGF21 plays a role in regulating adaptation to various metabolic abnormalities. In the liver, FGF21 plays an important role in the regulation of fatty acid oxidation both in the fasted state and in mice consuming a high-fat, low-carbohydrate ketogenic diet. FGF21 also regulates fatty acid metabolism in mice consuming a diet that promotes hepatic lipotoxicity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Fgf21, fibroblast growth factor 21 |