Mouse P-Selectin/CD62P ELISA Kit
Cat no : KE10075
Synonyms
CD62P, P selectin, P-Selectin, selectin, platelet, Selplg
Validation Data Gallery
Product Information
KE10075 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse P-Selectin/CD62P ELISA kit is to be used to detect and quantify protein levels of endogenous mouse P-Selectin/CD62P. The assay recognizes mouse P-Selectin/CD62P. An antibody specific for mouse P-Selectin/CD62P has been pre-coated onto the microwells. The mouse P-Selectin/CD62P protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse P-Selectin/CD62P is added to detect the captured mouse P-Selectin/CD62P protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse P-Selectin/CD62P ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Tissue lysates |
| Assay type | Sandwich |
| Sensitivity | 6.5 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20344 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 92% | 79%-102% |
| Tissue lysates | 105% | 96%-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 924.9 | 36.8 | 4.0 |
| 2 | 20 | 234.5 | 7.5 | 3.2 |
| 3 | 20 | 56.2 | 2.0 | 3.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 933.7 | 49.3 | 5.3 |
| 2 | 24 | 230.1 | 11.1 | 4.8 |
| 3 | 24 | 56.0 | 4.5 | 8.0 |
Background Information
P-sectieln, also named as SELP, GMP-140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface. Soluble P-selectin is produced by platelets and endothelial cells and circulates in normal plasma.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD62P, P selectin, P-Selectin, selectin, platelet, Selplg |