Mouse IgG3 isotype control Monoclonal antibody

Mouse IgG3 isotype control Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Megathura crenulata

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H4A5

Cat no : 66360-4-Ig

Synonyms

IgG3 isotype, mouse IgG control, Mouse IgG3 control, Mouse IgG3 isotype control

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Validation Data Gallery

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  WB analysis using 66360-4-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66360-4-Ig (KLH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected in

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66360-4-Ig targets Mouse IgG3 isotype control in WB, ELISA applications and shows reactivity with Megathura crenulata samples.

• Tested Reactivity: Megathura crenulata
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: Mouse IgG3 isotype control
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody is used as the isotype control of mouse IgG3. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).