Mouse IL-6 ELISA Kit

Sensitivity

3.8 pg/mL

Range

15.6-1000 pg/mL

Reactivity

Mouse

Cat no : KE10007

Synonyms

Il 6, Il6, IL-6, interleukin 6, Interleukin-6



Product Information

KE10007 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse IL6 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL6. The assay recognizes mouse IL6. An antibody specific for mouse IL6 has been pre-coated onto the microwells. The mouse IL6 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL6 is added to detect the captured mouse IL6 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Mouse IL-6 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 3.8 pg/mL
Range 15.6-1000 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA
Gene ID (NCBI) 16193

Recovery

Sample TypeAverageRange
Mouse serum99%75%-119%
Cell culture supernatants108%96%-123%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120133.36.14.5
220204.37.63.7
320778.152.76.8

InterAssay

Samplenmean ( pg/mL)SDCV%
124142.311.68.2
224223.018.88.4
324827.381.29.8

Background Information

Interleukin-6 (Il-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. Il-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. Il-6 Plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Il-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. Il-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, Il-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Il 6, Il6, IL-6, interleukin 6, Interleukin-6

Publications

SpeciesSample TypeTitle
mouseplasma

Nat Commun

Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents.

Authors - Ziwen Wang
mousetissue homogenates

Nucleic Acids Res

The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response.

Authors - Xin Zong
mouseplasma

J Am Soc Nephrol

Activation of Sympathetic Signaling in Macrophages Blocks Systemic Inflammation and Protects against Renal Ischemia-Reperfusion Injury.

Authors - Sho Hasegawa
mouseserum

Theranostics

Ultrasound (US)-activated redox dyshomeostasis therapy reinforced by immunogenic cell death (ICD) through a mitochondrial targeting liposomal nanosystem

Authors - Junjie Ren
mousecell culture supernatants

Environ Sci Technol

Chronic Exposure to Low Concentration of Graphene Oxide Increases Bacterial Pathogenicity via the Envelope Stress Response.

Authors - Qiurong Zhang
mouseplasma

Environ Sci Technol

Chronic Exposure to Titanium Dioxide Nanoparticles Induces Commensal-to-Pathogen Transition in Escherichia coli.

Authors - Qiurong Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Chintan (Verified Customer) (06-17-2019)

Primary microglia cells were isolated from mouse brain and treated with LPS. IL-6 concentrations were analysed using this kit. It worked as expected.

  • Applications: Western Blot,Cell culture,
  • Cell Tissue Type: primary microglia cells
FH

Joanna (Verified Customer) (06-07-2019)

The ELISA kit was used in order to detect changes between control and LPA (1µM) treated microglia cells. The kit was easy to use, including a very helpful and informative brochure. Results were consistent and I highly recommend this kit.

  • Applications: Cell culture,
  • Cell Tissue Type: BV2 microglia cells
FH

Joanna (Verified Customer) (06-07-2019)

The ELISA was used in order to detect changes in IL6 levels in control and LPA treated microglia cells. The kit was easy to use, including a detailed and very informative brochure. The results were consistent and this kit is highly recommended.

  • Applications: Cell culture,
  • Cell Tissue Type: BV2 microglia cells
FH

Chintan (Verified Customer) (06-03-2019)

This kit was used to estimate IL-6 levels in the cell culture medium of microglia cells. After stimulation with LPS, we observed elevated IL-6 production and excretion by microglia. This kit is easy to use. Standard curve and sample /positive control analysis worked perfectly.

  • Applications: Cell culture,
  • Cell Tissue Type: Mouse microglia