Mouse IL-4 ELISA Kit


1.0 pg/mL


7.8-500 pg/mL



Cat no : KE10010


BSF-1, Il 4, Il4, IL-4, interleukin 4, Interleukin-4

Product Information

KE10010 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse IL-4 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-4. The assay recognizes mouse IL-4. An antibody specific for mouse IL-4 has been pre-coated onto the microwells. The mouse IL-4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL-4 is added to detect the captured mouse IL-4 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Mouse IL-4 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 1.0 pg/mL
Range 7.8-500 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA
Gene ID (NCBI) 16189


Sample TypeAverageRange
Mouse serum89%72%-98%
Cell culture supernatants103%87%-117%


Samplenmean ( pg/mL)SDCV%


Samplenmean ( pg/mL)SDCV%

Background Information

Interleukin-4 (il-4), a member of the α-helical cytokine family, is produced by activated CD4+ T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. Il4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. Il4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.


Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms BSF-1, Il 4, Il4, IL-4, interleukin 4, Interleukin-4


SpeciesSample TypeTitle

Oxid Med Cell Longev

Simvastatin Reduces NETosis to Attenuate Severe Asthma by Inhibiting PAD4 Expression

Authors - Yun-Rong Chen
mousecell culture supernatants

Int Immunopharmacol

Nicotinamide mononucleotide attenuates airway epithelial barrier dysfunction via inhibiting SIRT3 SUMOylation in asthma

Authors - Jiayuan Liang
mousetissue homogenates

Biomed Pharmacother

Aster tataricus attenuates asthma efficiently by simultaneously inhibiting tracheal ring contraction and inflammation.

Authors - Yijun Chen
mousecell culture supernatants

Front Pharmacol

A Polysaccharide From the Whole Plant of Plantago asiatica L. Enhances the Antitumor Activity of Dendritic Cell-Based Immunotherapy Against Breast Cancer

Authors - Jiafeng Gao
mousecell culture supernatants

Front Pharmacol

Indole-3-Carbinol Selectively Prevents Chronic Stress-Induced Depression-but not Anxiety-Like Behaviors via Suppressing Pro-Inflammatory Cytokine Production and Oxido-Nitrosative Stress in the Brain.

Authors - Shengying Pan
mousetissue homogenates

Eur J Med Res

Research on the mechanism of prednisone in the treatment of ITP via VIP/PACAP-mediated intestinal immune dysfunction

Authors - Xiang Yan