Mouse IL-2 ELISA Kit
Cat no : KE10100
Synonyms
Il 2, Il2, interleukin 2
Validation Data Gallery
Product Information
KE10100 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL-2 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-2. The assay recognizes mouse IL-2. An antibody specific for mouse IL-2 has been pre-coated onto the microwells. The mouse IL-2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL-2 is added to detect the captured mouse IL-2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse IL-2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 4.5 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16183 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 80% | 71%-90% |
| Cell culture supernatant | 98% | 80%-106% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 765.1 | 27.2 | 3.5 |
| 2 | 20 | 188.5 | 8.6 | 4.6 |
| 3 | 20 | 41.9 | 3.2 | 7.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 836.7 | 40.6 | 4.8 |
| 2 | 24 | 187.4 | 8.2 | 4.4 |
| 3 | 24 | 43.1 | 4.5 | 10.5 |
Background Information
Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function and development, maintenance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signal is mediated through heterodimerization of the IL2Rβ and Il-2Rγ receptor subunits.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Il 2, Il2, interleukin 2 |