Mouse IL-12/IL-23 p40 ELISA Kit

Sensitivity

1.1 pg/mL

Range

15.6-1000 pg/mL

Reactivity

Mouse

Cat no : KE10068

Synonyms

Il 12b, Il 12p40, Il12b, Il12p40, interleukin 12b, p40



Product Information

KE10068 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL-12/IL-23 p40 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-12/IL-23 p40. The assay recognizes mouse IL-12/IL-23 p40. An antibody specific for mouse IL-12/IL-23 p40 has been pre-coated onto the microwells. The mouse IL-12/IL-23 p40 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL-12/IL-23 p40 is added to detect the captured mouse IL-12/IL-23 p40 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Mouse IL-12/IL-23 p40 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernates
Assay type Sandwich
Sensitivity 1.1 pg/mL
Range 15.6-1000 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA
Gene ID (NCBI) 16160

Recovery

Sample TypeAverageRange
Mouse serum93%75%-121%
Cell culture supernatants93%75%-109%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120427.018.24.3
220100.42.42.4
32027.90.83.0

InterAssay

Samplenmean ( pg/mL)SDCV%
124424.419.64.6
22495.43.53.7
32428.01.34.7

Background Information

IL-12p40 is known as a component of the bioactive cytokines interleukin IL-12 and IL-23, which can exist in a monomeric or homodimeric form. IL-12p40 is produced primarily by activated inflammatory cells including macrophages, neutrophils, microglia and dendritic cells (DCs). The most widely appreciated function of IL-12p40 is to provide a negative feedback loop by competitively binding to the IL-12 receptor. Additionally, IL-12p40 can also act as a chemoattractant for macrophages and promote the migration of bacterially stimulated dendritic cells. It is also associated with several pathogenic inflammatory responses such as silicosis, graft rejection.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Il 12b, Il 12p40, Il12b, Il12p40, interleukin 12b, p40

Publications

SpeciesSample TypeTitle
mousecell culture supernatants

J Nanobiotechnology

Photosensitive and dual-targeted chromium nanoparticle delivering small interfering RNA YTHDF1 for molecular-targeted immunotherapy in liver cancer

Authors - Shang Chen