Mouse IL-10 ELISA Kit

Sensitivity

3.0 pg/mL

Range

31.25-2000 pg/mL

Reactivity

Mouse

Cat no : KE10103

Synonyms

CSIF, Il 10, Il10, interleukin 10



Product Information

KE10103 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL-10 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-10. The assay recognizes mouse IL-10. An antibody specific for mouse IL-10 has been pre-coated onto the microwells. The mouse IL-10 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL-10 is added to detect the captured mouse IL-10 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Mouse IL-10 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatant
Assay type Sandwich
Sensitivity 3.0 pg/mL
Range 31.25-2000 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA
Gene ID (NCBI) 16153

Recovery

Sample TypeAverageRange
Mouse serum88%74%-124%
Cell culture supernatant109%89%-121%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120695.333.84.9
220166.39.95.9
32033.22.78.3

InterAssay

Samplenmean ( pg/mL)SDCV%
124652.723.23.6
224160.57.24.5
32439.52.66.7

Background Information

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms CSIF, Il 10, Il10, interleukin 10

Publications

SpeciesSample TypeTitle
mouseserum

Phytomedicine

Preparation and characterisation of baicalin magnesium and its protective effect in ulcerative colitis via gut microbiota-bile acid axis modulation

Authors - Lin Zhang
tissue homogenates

Int J Mol Sci

Sustained Effectiveness and Safety of Therapeutic miR-10a/b in Alleviating Diabetes and Gastrointestinal Dysmotility without Inducing Cancer or Inflammation in Murine Liver and Colon

Authors - Rajan Singh
mouse

Int J Mol Sci

Immunoregulatory Effects of Elemental Diet and Its Ingredient, Tryptophan, via Activation of the Aryl Hydrocarbon Receptor in Mice

Authors - Atsuhito Kubota
mouseserum

Stem Cells

Fructose Potentiates Bone Loss and Marrow Adipose Tissue Accumulation by Inhibiting Adenosine 5'-Monophosphate-Activated Protein Kinase in Mesenchymal Stem Cells

Authors - Ziqi Yan
mousecell lysates

J Nanobiotechnology

A novel microglia-targeting strategy based on nanoparticle-mediated delivery of miR-26a-5p for long-lasting analgesia in chronic pain

Authors - Yitian Lu
mousetissue homogenates

BMC Microbiol

Clostridium butyricum inhibits the inflammation in children with primary nephrotic syndrome by regulating Th17/Tregs balance via gut-kidney axis

Authors - Ting Li