Mouse IL-1 beta ELISA Kit
Cat no : KE10003
Synonyms
Il 1b, IL 1beta, IL-1, IL1 BETA, IL-1 beta, Il1b, IL-1b, IL1beta, IL1β, interleukin 1 beta
Validation Data Gallery
Product Information
KE10003 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL1-beta ELISA kit is to be used to detect and quantify protein levels of endogenous IL1-beta. The assay recognizes mouse IL1-beta. An antibody specific for IL1-beta has been pre-coated onto the microwells. The IL1-beta protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL1-beta is added to detect the captured IL1-beta protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.NB: precursor and active form can be detected by this kit.
Product name | Mouse IL-1 beta ELISA Kit |
Tests | 1 X 96 well plate |
Sample type | Serum, Plasma, Cell culture supernatants, tissue lysates |
Assay type | Sandwich |
Sensitivity | 1.0 pg/mL |
Range | 7.8-500 pg/mL |
Reactivity | Mouse |
Tested applications | Sandwich ELISA |
Gene ID (NCBI) | 16176 |
Recovery
Sample Type | Average | Range |
---|---|---|
Mouse serum | 102% | 91%-123% |
Cell culture supernatants | 113% | 89%-126% |
Cell lysates | 104% | 82%-128% |
IntraAssay
Sample | n | mean ( pg/mL) | SD | CV% |
---|---|---|---|---|
1 | 20 | 520.0 | 17.0 | 3.3 |
2 | 20 | 159.5 | 5.6 | 3.5 |
3 | 20 | 29.1 | 0.5 | 1.8 |
InterAssay
Sample | n | mean ( pg/mL) | SD | CV% |
---|---|---|---|---|
1 | 24 | 485.5 | 20.1 | 4.1 |
2 | 24 | 156.6 | 4.8 | 3.1 |
3 | 24 | 28.5 | 1.3 | 4.6 |
Background Information
Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor IL-1RN. Interleukin 1β(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions.
Properties
Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
Synonyms | Il 1b, IL 1beta, IL-1, IL1 BETA, IL-1 beta, Il1b, IL-1b, IL1beta, IL1β, interleukin 1 beta |
Publications
Species | Sample Type | Title |
---|---|---|
Nat Commun Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH) | ||
Nat Commun Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents. | ||
Cell Mol Immunol NLRP3-dependent pyroptosis is required for HIV-1 gp120-induced neuropathology. | ||
Environ Sci Technol Chronic Exposure to Low Concentration of Graphene Oxide Increases Bacterial Pathogenicity via the Envelope Stress Response. | ||
Cancer Lett SIGLEC15 amplifies immunosuppressive properties of tumor-associated macrophages in pancreatic cancer. | ||
Carbohydr Polym Cyclodextrin boostered-high density lipoprotein for antiatherosclerosis by regulating cholesterol efflux and efferocytosis. |