Mouse/Rat FGF-21 ELISA Kit


0.4 pg/mL


15.6-1000 pg/mL



Cat no : KE10042


Fgf21, FGF-21, fibroblast growth factor 21

Product Information

KE10042 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse/Rat FGF-21 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse/rat FGF-21. The assay recognizes mouse/rat FGF-21. An antibody specific for mouse/rat FGF-21 has been pre-coated onto the microwells. The mouse/rat FGF-21 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse/rat FGF-21 is added to detect the captured mouse/rat FGF-21 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Mouse/Rat FGF-21 ELISA Kit
Tests 1 X 96 well plate
Sample type Mouse serum, Mouse plasma,Rat serum, Rat plasma,Cell culture supernatants
Assay type Sandwich
Sensitivity 0.4 pg/mL
Range 15.6-1000 pg/mL
Reactivity Mouse/Rat
Tested applications Sandwich ELISA
Gene ID (NCBI) 56636


Sample TypeAverageRange
Mouse serum87%75%-123%
Rat serum86%73%-110%
Cell culture supernatants87%79%-104%


Samplenmean ( pg/mL)SDCV%


Samplenmean ( pg/mL)SDCV%

Background Information

Fibroblast growth factor 21 (FGF21) is a peptide hormone that is expressed across several tissues, predominantly in liver and adipocyte tissues, and, to a lower extent, in the pancreas, skeletal muscle, heart, kidneys and testes. FGF21 plays a role in regulating adaptation to various metabolic abnormalities. In the liver, FGF21 plays an important role in the regulation of fatty acid oxidation both in the fasted state and in mice consuming a high-fat, low-carbohydrate ketogenic diet. FGF21 also regulates fatty acid metabolism in mice consuming a diet that promotes hepatic lipotoxicity.


Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Fgf21, FGF-21, fibroblast growth factor 21


SpeciesSample TypeTitle

Sci Adv

Mitochondrial proteostasis stress in muscle drives a long-range protective response to alleviate dietary obesity independently of ATF4.

Authors - Qiqi Guo
mousecell culture supernatants

Front Pharmacol

Elucidation of the Mechanism of Action of Ginseng Against Acute Lung Injury/Acute Respiratory Distress Syndrome by a Network Pharmacology-Based Strategy.

Authors - Qi Ding
mousecell culture supernatants

Biol Pharm Bull

Nobiletin and 3'-Demethyl Nobiletin Activate Brown Adipocytes upon β-Adrenergic Stimulation.

Authors - Fumiko Kihara-Negishi

Cell Rep

The autophagy protein Becn1 improves insulin sensitivity by promoting adiponectin secretion via exocyst binding.

Authors - Kenta Kuramoto

Pediatr Res

FGF21 modulates hippocampal cold-shock proteins and CA2-subregion proteins in neonatal mice with hypoxia-ischemia

Authors - Jeremy R Herrmann