Mouse CCL3 ELISA Kit
Cat no : KE10023
Synonyms
AI323804, Ccl3, G0S19 1, LD78alpha, LD78-alpha, MIP 1alpha, MIP1 (a), MIP1 alpha, Mip1a, MIP-1-alpha, Scya3, SIS-beta
Validation Data Gallery
Product Information
KE10023 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse CCL3 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CCL3. The assay recognizes mouse CCL3. An antibody specific for mouse CCL3 has been pre-coated onto the microwells. The mouse CCL3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse CCL3 is added to detect the captured mouse CCL3 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CCL3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.03 pg/mL |
| Range | 1.95-125 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20302 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 107% | 75%-126% |
| Cell culture supernatants | 104% | 87%-123% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 86.8 | 3.8 | 4.4 |
| 2 | 20 | 25.8 | 0.9 | 3.5 |
| 3 | 20 | 5.3 | 0.1 | 2.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 83.8 | 3.9 | 4.7 |
| 2 | 24 | 25.7 | 0.8 | 3.0 |
| 3 | 24 | 5.1 | 0.3 | 5.4 |
Background Information
Chemokine (C-C motif) ligand 3 (CCL3), also known as MIP-1 alpha, belongs to the family of chemokines. CCL3 has been found in the central nervous system and its cognate receptors, CCR1 and CCR5, have been reported to be expressed by astrocytes, microglia and neurons. CCL3 and its receptors, CCR1 and CCR5, also contribute to the development of bone disease in multiple myeloma by supporting tumor growth and regulating osteoclast differentiation. CCL3 is also associated with the regulation of cell growth, angiogenesis, and metastasis of different tumors such as melanoma, renal cell carcinoma, and colorectal cancer. Moreover, CCL3 enhances cell migration and metastasis by up-regulating matrix metalloproteinase-2 (MMP)-2 expression in chondrosarcoma cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AI323804, Ccl3, G0S19 1, LD78alpha, LD78-alpha, MIP 1alpha, MIP1 (a), MIP1 alpha, Mip1a, MIP-1-alpha, Scya3, SIS-beta |
Publications
| Species | Sample Type | Title |
|---|---|---|
Biomed Pharmacother Aster tataricus attenuates asthma efficiently by simultaneously inhibiting tracheal ring contraction and inflammation. |