Mono-Methyl-Histone H3 (Lys9) Recombinant antibody

Mono-Methyl-Histone H3 (Lys9) Uni-rAb^TM Recombinant Antibody for WB, Dot Blot, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, Dot Blot, ELISA

Conjugate

Unconjugated

CloneNo.

3C23

Cat no : 82821-6-RR

Synonyms

HIST1H3A, 3C23, Histone-H3, HistoneH3, Histone H3

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Validation Data Gallery

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  WB analysis using 82821-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82821-6-RR (Mono-Methyl-Histone H3 (Lys9) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Dot Blot experiment of peptide using 82821-6-RR

  Dot blot analysis was used to confirm the specificity of 82821-6-RR Mono-Methyl-Histone H3 (Lys9) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, K-562 cells, THP-1 cells, HSC-T6 cells
• Positive Dot Blot detected in: peptide

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• DOT BLOT: DOT BLOT : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82821-6-RR targets Mono-Methyl-Histone H3 (Lys9) in WB, Dot Blot, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: histone cluster 1, H3a
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC066245
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified. Methylation of Histone H3 at lysine 9 is linked to transcriptional repression.