Mono/Di-Methyl-Histone H3 (Lys9) Recombinant antibody

Mono/Di-Methyl-Histone H3 (Lys9) Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E12

Cat no : 80219-1-RR

Synonyms

HIST1H3A, 1E12, Histone-H3, HistoneH3, Histone H3

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Validation Data Gallery

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  WB analysis using 80219-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80219-1-RR (Mono/Di-Methyl-Histone H3 (Lys9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 80219-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Mono/Di-Methyl-Histone H3 (Lys9) antibody (80219-1-RR, Clone: 1E12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HeLa using 80219-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Mono/Di-Methyl-Histone H3 (Lys9) antibody (80219-1-RR, Clone: 1E12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: THP-1 cells, K-562 cells, Jurkat cells, HeLa cells, HEK-293 cells, NIH/3T3 cells, C2C12 cells, HSC-T6 cells, PC-12 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

80219-1-RR targets Mono/Di-Methyl-Histone H3 (Lys9) in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: histone cluster 1, H3a
• Observed Molecular Weight: 15-18 kDa
• GenBank Accession Number: BC066245
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• RRID: AB_2918878
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified. Methylation of Histone H3 at lysine 9 is linked to transcriptional repression. This antibody is specific to monomethyl or dimethyl-Histone H3 while it does not recognize trimethyl-Histone H3 (Lys9). It is also named as H3K9me1/2.