Mlkl Polyclonal antibody

Mlkl Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 30145-1-AP

Synonyms

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Validation Data Gallery

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  WB analysis using 30145-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30145-1-AP (Mlkl antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30145-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30145-1-AP (Mlkl antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 30145-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Mlkl antibody (30145-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of NIH/3T3 using 30145-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Mlkl antibody (30145-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: 4T1 cells, HSC-T6 cells, NIH/3T3 cells
• Positive IF/ICC detected in: NIH/3T3 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

30145-1-AP targets Mlkl in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Mlkl fusion protein Ag32514
• Full Name: mixed lineage kinase domain-like
• Calculated Molecular Weight: 54kd
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: NM_001310613
• Gene Symbol: Mlkl
• Gene ID (NCBI): 74568
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mixed lineage kinase domain-like pseudokinase (Mlkl) belongs to the protein kinase superfamily and has two MWs of 54 and 30 kDa. Mlkl plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in the necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children.

Publications

Species	Application	Title
mouse	WB	FASEB J; Metformin switches cell death modes to soothe the apical periodontitis via ZBP1; Authors - Hui Liu