MECR Polyclonal antibody

MECR Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 51027-2-AP

Synonyms

Nuclear receptor-binding factor 1, NRBF-1, Nrbf1, Enoyl-[acyl-carrier-protein] reductase, mitochondrial, EC:1.3.1.104

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Validation Data Gallery

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  WB analysis using 51027-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51027-2-AP (MECR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 51027-2-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 51027-2-AP (MECR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 51027-2-AP

  IP result of anti-MECR (IP:51027-2-AP, 4ug; Detection:51027-2-AP 1:1500) with mouse heart tissue lysate 4800ug.

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  IF Staining of C2C12 using 51027-2-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using MECR antibody (51027-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse skeletal muscle tissue, mouse stomach tissue, rat heart tissue
• Positive IP detected in: mouse heart tissue
• Positive IF/ICC detected in: C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

51027-2-AP targets MECR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, yeast
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MECR fusion protein Ag0465
• Full Name: mitochondrial trans-2-enoyl-CoA reductase
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 37-40 kDa
• GenBank Accession Number: BC003864
• Gene Symbol: MECR
• Gene ID (NCBI): 26922
• RRID: AB_615013
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mitochondrial Trans-2-Enoyl-CoA Reductase is abbrivated as MECR, involved in human mitochonrial fatty acid synthesis(mtFAS) as an oxidoreductase. (PMID: 27817865) MECR has molecular weight can be calculated around 37KDa. With immunopercipitation reaction on mouse heart samples, the IgG heavy chain, dimer of MECR is around 65KDa. Numerous studies have shown disorder of lipid metabolism is closely related with malignance, especially in liver cancer. As futher research suggests that MECR has close realitionship with cell prolifereation, appotosis, and metastasis (PMID: 31178528)

Publications

Species	Application	Title
human	WB	Am J Hum Genet; MECR Mutations Cause Childhood-Onset Dystonia and Optic Atrophy, a Mitochondrial Fatty Acid Synthesis Disorder.; Authors - Gali Heimer
mouse	WB	Elife; Mitochondrial fatty acid synthesis coordinates oxidative metabolism in mammalian mitochondria.; Authors - Sara M Nowinski
mouse	IHC,IF	J Neurosci; Impaired mitochondrial fatty acid synthesis leads to neurodegeneration in mice.; Authors - Remya R Nair
mouse	WB	Hum Mol Genet; Genetic modifications of Mecr reveal a role for mitochondrial 2-enoyl-CoA/ACP reductase in placental development in mice.; Authors - Remya R Nair
yeast	WB	Nat Commun; An engineered variant of MECR reductase reveals indispensability of long-chain acyl-ACPs for mitochondrial respiration; Authors - M Tanvir Rahman
mouse	IHC,IF	Lipids Health Dis; A novel lipid metabolism-based risk model associated with immunosuppressive mechanisms in diffuse large B-cell lymphoma; Authors - Zhaoli Zhang