MECR Polyclonal antibody

• Featured Product
• KD/KO Validated

MECR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 51025-2-Ig

Synonyms

Nuclear receptor-binding factor 1, NRBF-1, Nrbf1, Enoyl-[acyl-carrier-protein] reductase, mitochondrial, EC:1.3.1.104

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 51025-2-Ig

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 51025-2-Ig (MECR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 51025-2-Ig

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 51025-2-Ig (MECR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 51025-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 51025-2-Ig (MECR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 51025-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 51025-2-Ig (MECR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, mouse heart tissue
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

51025-2-Ig targets MECR in WB, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: mitochondrial trans-2-enoyl-CoA reductase
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC003864
• Gene Symbol: MECR
• Gene ID (NCBI): 26922
• RRID: AB_1167469
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mitochondrial Trans-2-Enoyl-CoA Reductase is abbrivated as MECR, involved in human mitochonrial fatty acid synthesis(mtFAS) as an oxidoreductase. (PMID: 27817865) MECR has molecular weight can be calculated around 37KDa. With immunopercipitation reaction on mouse heart samples, the IgG heavy chain, dimer of MECR is around 65KDa. Numerous studies have shown disorder of lipid metabolism is closely related with malignance, especially in liver cancer. As futher research suggests that MECR has close realitionship with cell prolifereation, appotosis, and metastasis (PMID: 31178528)

Publications

Species	Application	Title
mouse	WB	Redox Biol; Non-electron transfer chain mitochondrial defects differently regulate HIF-1α degradation and transcription.; Authors - Antonina N Shvetsova ; KD Validated
mouse	WB	Biochem Biophys Res Commun; The mitochondrial fatty acid synthesis (mtFASII) pathway is capable of mediating nuclear-mitochondrial cross talk through the PPAR system of transcriptional activation.; Authors - Parl Angelika A