MCP-1/CCL2 Polyclonal antibody

MCP-1/CCL2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

22

Cat no : 26161-1-AP

Synonyms

Ccl2, MCP1, MCP-1, Chemokine (CC motif) ligand 2, chemokine (C C motif) ligand 2

---

Validation Data Gallery

• 

  WB analysis using 26161-1-AP

  Untreated RAW 264.7 cells, RAW 264.7 cells (treated with 1 μg/ml LPS 20 hours then add 300 ng/ml Brefeldin A for 4hours) were subjected to SDS PAGE followed by western blot with 26161-1-AP (Mcp1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 26161-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 26161-1-AP (Mcp1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: LPS and Brefeldin A treated RAW 264.7 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 13 publications below
• IHC: See 5 publications below
• IF: See 9 publications below

Product Information

26161-1-AP targets MCP-1/CCL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MCP-1/CCL2 fusion protein Ag24085
• Full Name: chemokine (C-C motif) ligand 2
• Observed Molecular Weight: 16-20 kDa
• GenBank Accession Number: NM-011333
• Gene Symbol: MCP-1/CCL2
• Gene ID (NCBI): 20296
• RRID: AB_2918100
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Monocyte chemoattractant protein-1 (MCP-1/CCL2) is one of the key chemokines that regulate migration and infiltration of monocytes/macrophages. Both CCL2 and its receptor CCR2 have been demonstrated to be induced and involved in various diseases. Migration of monocytes from the blood stream across the vascular endothelium is required for routine immunological surveillance of tissues, as well as in response to inflammation.

Publications

Species	Application	Title
mouse	WB,IHC,IF	Acta Pharmacol Sin; Macrophages promote the transition from myocardial ischemia reperfusion injury to cardiac fibrosis in mice through GMCSF/CCL2/CCR2 and phenotype switching; Authors - Shi-Chun Shen
rat	WB	Int J Mol Sci; Enhanced Activation of the S1PR2-IL-1β-Src-BDNF-TrkB Pathway Mediates Neuroinflammation in the Hippocampus and Cognitive Impairment in Hyperammonemic Rats; Authors - María Sancho-Alonso
mouse	IF	Front Cell Neurosci; Effects of Ischemia on the Migratory Capacity of Microglia Along Collagen Microcontact Prints on Organotypic Mouse Cortex Brain Slices.; Authors - Katharina Steiner
mouse	IHC	Pharmaceutics; Targeted Therapy of Acute Liver Injury via Cryptotanshinone-Loaded Biomimetic Nanoparticles Derived from Mesenchymal Stromal Cells Driven by Homing; Authors - Xin Zhang
mouse	IF	J Biol Chem; rhFGF-21 accelerates corneal epithelial wound healing through the attenuation of oxidative stress and inflammatory mediators in diabetic mice; Authors - Le Li
mouse	IF	Proc Natl Acad Sci U S A; Bacterial indole-3-lactic acid affects epithelium-macrophage crosstalk to regulate intestinal homeostasis; Authors - Kaiyuan Yu