MYST2 Monoclonal antibody

MYST2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C1B1

Cat no : 68525-1-Ig

Synonyms

HBO1, HBOA, KAT7, MYST 2, MYST2

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Validation Data Gallery

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  WB analysis using 68525-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68525-1-Ig (MYST2 antibody) at dilution of 1:4900 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, LNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68525-1-Ig targets MYST2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MYST2 fusion protein Ag5166
• Full Name: MYST histone acetyltransferase 2
• Calculated Molecular Weight: 611 aa, 71 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC032640
• Gene Symbol: MYST2
• Gene ID (NCBI): 11143
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYST2, also named as HBO1, HBOa MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2, belongs to the MYST (SAS/MOZ) family. It specifically represses AR mediated transcription. MYST2 is a candidate oncogene. It enhances the anchorage-independent growth of breast cancer cells.(PMID:19372580) MYST2 is a histone acetyltransferase (HAT) which could exert oncogenic function in breast cancer. It is an important downstream molecule of ERa, and ERK1/2 signaling pathway may involved in the expression of HBO1 increased by E2.