MYPT1 Monoclonal antibody, PBS Only

MYPT1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human, rat, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A1A9

Cat no : 66506-1-PBS

Synonyms

MBS, MYPT1, PPP1R12A

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Validation Data Gallery

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  WB analysis of Raji using 66506-1-Ig (same clone as 66506-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66506-1-Ig (MYPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66506-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 66506-1-Ig (same clone as 66506-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66506-1-Ig (MYPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66506-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66506-1-Ig (same clone as 66506-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66506-1-Ig (MYPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66506-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66506-1-Ig (same clone as 66506-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66506-1-Ig (MYPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66506-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66506-1-Ig (same clone as 66506-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66506-1-Ig (MYPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66506-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66506-1-Ig (same clone as 66506-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MYPT1 antibody (66506-1-Ig, Clone: 2A1A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66506-1-PBS in a different storage buffer formulation.

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Product Information

66506-1-PBS targets MYPT1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: MYPT1 fusion protein Ag17496
• Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 12A
• Calculated Molecular Weight: 1030 aa, 115 kDa
• Observed Molecular Weight: 115 kDa
• GenBank Accession Number: BC111752
• Gene Symbol: MYPT1
• Gene ID (NCBI): 4659
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Myosin phosphatase target subunit 1(MYPT1), which is also called PPP1R12A, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization.