MYOT Polyclonal antibody

MYOT Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 10731-1-AP

Synonyms

57 kDa cytoskeletal protein, LGMD1, LGMD1A, MYOT, myotilin, TTID

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 10731-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10731-1-AP (MYOT antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10731-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10731-1-AP (MYOT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10731-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10731-1-AP (MYOT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 10731-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10731-1-AP (MYOT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 10731-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10731-1-AP (MYOT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, mouse heart tissue
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

10731-1-AP targets MYOT in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYOT fusion protein Ag1112
• Full Name: myotilin
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55-57 kDa, 35 kDa
• GenBank Accession Number: BC005376
• Gene Symbol: MYOT
• Gene ID (NCBI): 9499
• RRID: AB_2297956
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYOT (myotilin) is a structural protein of the striated muscle Z-discs. It interacts with both actinin and filamin, forming a complex to maintain structural stability of muscles. In adult tissues, myotilin is mainly expressed in skeletal and cardiac muscles and in the peripheral nerves. Missense mutations of myotilin cause limb girdle muscular dystrophy 1A and some other myopathy. Two isoforms of MYOT exist due to the alternative splicing. This antibody can detect both of isoforms around 57 kDa and 35 kDa.

Publications

Species	Application	Title
human	IHC	Neurology; Mutations in HSPB8 causing a new phenotype of distal myopathy and motor neuropathy.; Authors - Roula Ghaoui
human	WB	Am J Pathol; In vivo characterization of mutant myotilins.; Authors - Keduka Etsuko E
		Neurol Genet; Novel mutation in TNPO3 causes congenital limb-girdle myopathy with slow progression.; Authors - Anna Vihola
mouse	IHC	Elife; Isoform-specific mutation in Dystonin-b gene causes late-onset protein aggregate myopathy and cardiomyopathy; Authors - Nozomu Yoshioka
human	IF	Eur J Neurol; Novel mutations in DNAJB6 cause LGMD1D and distal myopathy in French families; Authors - P H Jonson
human	IHC	Neurol Neuroimmunol Neuroinflamm; Clinicopathologic Profiles of Sporadic Late-Onset Nemaline Myopathy: Practical Importance of Anti-α-Actinin Immunostaining; Authors - Bing Zhao