MYOM1-Specific Polyclonal antibody

MYOM1-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, RIP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 20360-1-AP

Synonyms

MYOM1, Myomesin-1, Myomesin family member 1, Myomesin 1, 190 kDa titin-associated protein

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Validation Data Gallery

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  WB analysis using 20360-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 20360-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 20360-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20360-1-AP (MYOM1-Specific Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 20360-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20360-1-AP (MYOM1-Specific Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 20360-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20360-1-AP (MYOM1-Specific Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 20360-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20360-1-AP (MYOM1-Specific Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 20360-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 20360-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse skeletal muscle using 20360-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 20360-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 20360-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 20360-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of C2C12 using 20360-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using MYOM1-Specific antibody (20360-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue
• Positive IHC detected in: human skeletal muscle tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse skeletal muscle tissue, mouse heart tissue
• Positive IF/ICC detected in: C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• RIP: See 1 publications below

Product Information

20360-1-AP targets MYOM1-Specific in WB, IHC, IF/ICC, IF-P, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: myomesin 1, 185kDa
• Calculated Molecular Weight: 188 kDa
• Observed Molecular Weight: 190 kDa
• GenBank Accession Number: NM_003803
• Gene Symbol: MYOM1
• Gene ID (NCBI): 8736
• RRID: AB_10696312
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYOM1 is a major component of the vertebrate myofibrillar M band. MYOM1 binds myosin, titin, and light meromyosin. This binding is dose-dependent. The antibody is specific to MYOM1.

Publications

Species	Application	Title
mouse	WB	Sci Rep; Hypertension reduces soluble guanylyl cyclase expression in the mouse aorta via the Notch signaling pathway.; Authors - Catarina Rippe
mouse	WB	J Cell Mol Med; Hydrogen sulphide ameliorating skeletal muscle atrophy in db/db mice via Muscle RING finger 1 S-sulfhydration.; Authors - Fangping Lu
mouse	WB,RIP	iScience; Splicing factor Srsf5 deletion disrupts alternative splicing and causes noncompaction of ventricular myocardium; Authors - Xiaoli Zhang, Ze
rat	IHC,IF	Cell Signal; Long non-coding RNA Pvt1 modulates the pathological cardiac hypertrophy via miR-196b-mediated OSMR regulation.; Authors - Qingqing Wu
rat	IF	Int J Mol Sci; Skeletal Muscle Dysfunction in Experimental Pulmonary Hypertension; Authors - Kosmas Kosmas