MYOF Recombinant antibody

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  FC experiment of HepG2 using 82917-1-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human MYOF (82917-1-RR, Clone:230213C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of HeLa using 82917-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human MYOF (82917-1-RR, Clone:230213C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

MYOF Uni-rAb^TM Recombinant Antibody for FC (Intra), ELISA

Cat No. 82917-1-RR

Clone No.230213C6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Synonyms

Myoferlin, KIAA1207, Fer-1-like protein 3, FER1L3, Fer 1 like protein 3

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Tested Applications

• Positive FC (Intra) detected in: HepG2 cells, HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82917-1-RR targets MYOF in FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: myoferlin
• Calculated Molecular Weight: 235 kDa
• GenBank Accession Number: NM_133337
• Gene Symbol: MYOF
• Gene ID (NCBI): 26509
• RRID: AB_3670651
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NZM1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYOF, also named FER1L3 and KIAA1207, belongs to the Ferlin family. MYOF is a calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. It is involved in endocytic recycling. MYOF is implicated in VEGF signal transduction by regulating the levels of the receptor KDR.