Myocilin Polyclonal antibody

Myocilin Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 14238-1-AP

Synonyms

GLC1A, GPOA, JOAG, JOAG1, MYOC, Myocilin, TIGR

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Validation Data Gallery

• 

  WB analysis of human heart using 14238-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14238-1-AP (Myocilin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14238-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14238-1-AP (Myocilin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 14238-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 14238-1-AP (Myocilin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 14238-1-AP

  IP result of anti-Myocilin (IP:14238-1-AP, 4ug; Detection:14238-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: human heart tissue, human stomach tissue, mouse skeletal muscle tissue
• Positive IP detected in: mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

14238-1-AP targets Myocilin in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Myocilin fusion protein Ag5492
• Full Name: myocilin, trabecular meshwork inducible glucocorticoid response
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC029261
• Gene Symbol: Myocilin
• Gene ID (NCBI): 4653
• RRID: AB_2148652
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myocilin (MYOC), also known as TIGR or GLC1A, is a secreted glycoprotein of the olfactomedin family, originally identified in trabecular meshwork cells after prolonged treatment with glucocorticoids, and, independently, in the retina (PMID: 9176893; 9169133). It may participate in the obstruction of fluid outflow in the trabecular meshwork. Defects in MYOC are the cause of primary open angle glaucoma (POAG) (PMID: 21709622). MYOC has a molecular weight of 55-57 kDa, besides, a 66-kDa form, which could be a result of post-translational modifications, has also been reported in some researches (PMID: 9497363; 12150989).

Publications

Species	Application	Title
human	WB	Ann Transl Med; Adenosine A3 receptor activated in H2O2 oxidative stress of primary open-angle glaucoma.; Authors - Ziyu Zhou
human	WB	Int J Ophthalmol; Expression profile analysis to identify potential gene changes induced by dexamethasone in the trabecular meshwork; Authors - Miao Wei
mouse	WB	J Ocul Pharmacol Ther; Activation of Sonic Hedgehog Signaling Pathway Regulates Human Trabecular Meshwork Cell Function; Authors - Xiaochen Wang
human	WB	Int J Mol Sci; The Delayed Turnover of Proteasome Processing of Myocilin upon Dexamethasone Stimulation Introduces the Profiling of Trabecular Meshwork Cells' Ubiquitylome; Authors - Grazia Raffaella Tundo