MYO9B Recombinant antibody

MYO9B Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241163D8

Cat no : 84090-4-RR

Synonyms

Unconventional myosin-9b, MYR5, myosin IXB, MYO9B variant protein, CELIAC4

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Validation Data Gallery

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  WB analysis using 84090-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84090-4-RR (MYO9B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of A431 using 84090-4-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug MYO9B Recombinant antibody (84090-4-RR, Clone:241163D8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: K-562 cells, Jurkat cells, HL-60 cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84090-4-RR is MYO9B Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MYO9B fusion protein Ag33349
• Full Name: myosin IXB
• Calculated Molecular Weight: 2023 aa, 223 kDa
• Observed Molecular Weight: 230-250 kDa
• GenBank Accession Number: BC018108
• Gene Symbol: MYO9B
• Gene ID (NCBI): 4650
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.