MYO7A Recombinant antibody

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  WB analysis using 83807-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83807-1-RR (MYO7A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of Y79 using 83807-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Y79 cells using MYO7A antibody (83807-1-RR, Clone: 240612C11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

MYO7A Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 83807-1-RR

Clone No.240612C11

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, ELISA

Synonyms

MYU7A, MYOVIIA, myosin VIIA, DFNB2, DFNA11

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Tested Applications

• Positive WB detected in: A431 cells, Y79 cells, rat retina tissue
• Positive IF/ICC detected in: Y79 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83807-1-RR targets MYO7A in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: myosin VIIA
• Calculated Molecular Weight: 254 kDa
• Observed Molecular Weight: 254 kDa
• GenBank Accession Number: NM_000260
• Gene Symbol: MYO7A
• Gene ID (NCBI): 4647
• RRID: AB_3671394
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q13402
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYO7A, also named a USH1B, is one of myosins protein which are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. In retina, MYO7A might play a role in trafficking of ribbon-synaptic vesicle complexes and renewal of the outer photoreceptors disks. In inner ear, it might maintain the rigidity of stereocilia during the dynamic movements of the bundle. It is involved in hair-cell vesicle trafficking of aminoglycosides, which are known to induce ototoxicity. Defects in MYO7A are the cause of Usher syndrome type 1B (USH1B). Defects in MYO7A are the cause of deafness autosomal recessive type 2 (DFNB2). Defects in MYO7A are the cause of deafness autosomal dominant type 11 (DFNA11). The antibody is specific to MYO7A.