MYO6 Recombinant antibody

• 

  WB analysis using 84085-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84085-3-RR (MYO6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 84085-3-RR

  LNCaP cells were subjected to SDS PAGE followed by western blot with 84085-3-RR (MYO6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

MYO6 {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 84085-3-RR

Clone No.241251G11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Unconventional myosin 6, myosin VI, KIAA0389, DFNB37, DFNA22

---

Tested Applications

• Positive WB detected in: MCF-7 cells, LNCaP cells, HEK-293T cells, DU 145 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84085-3-RR targets MYO6 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MYO6 fusion protein Ag24906
• Full Name: myosin VI
• Calculated Molecular Weight: 1285 aa, 149 kDa
• Observed Molecular Weight: 145-150 kDa
• GenBank Accession Number: BC146764
• Gene Symbol: MYO6
• Gene ID (NCBI): 4646
• RRID: AB_3671653
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UM54
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYO6, an actin-based motor protein, is the only myosin known to move toward the minus end of actin filaments. MYO6 is highly expressed in the inner and outer hair cells of the ear, retina, and polarized epithelial cells such as kidney proximal tubule cells and intestinal enterocytes. And it participates in a wide range of biological processes within cells, including clathrin-mediated endocytosis, vesicular membrane traffic, polarized secretion, and autophagy (PMID: 23620821; PMID: 28591580). Previous studies showed that MYO6 is upregulated in various types of cancer, and it has been widely reported to contribute to tumor cell migration and metastasis. Some articles indicate that MYO6 is associated with prostate cancer, lung cancer, human colorectal cancer and gastric cancer (PMID: 29022908).