MYO6 Polyclonal antibody

• Featured Product
• KD/KO Validated

MYO6 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (2)

Cat no : 26778-1-AP

Synonyms

Unconventional myosin-6, Unconventional myosin 6, myosin VI, KIAA0389, DFNB37

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Validation Data Gallery

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  WB analysis of mouse small intestine using 26778-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 26778-1-AP (MYO6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 26778-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26778-1-AP (MYO6 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 26778-1-AP

  IP result of anti-MYO6 (IP:26778-1-AP, 4ug; Detection:26778-1-AP 1:500) with PC-3 cells lysate 2000 ug.

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  IHC staining of human prostate cancer using 26778-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26778-1-AP (MYO6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 26778-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 26778-1-AP (MYO6 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse small intestine using 26778-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using 26778-1-AP (MYO6 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 26778-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MYO6 antibody (26778-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse small intestine tissue, DU 145 cells, LNCaP cells, MCF-7 cells
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: human prostate cancer tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse small intestine tissue
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

26778-1-AP targets MYO6 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYO6 fusion protein Ag24906
• Full Name: myosin VI
• Calculated Molecular Weight: 1285 aa, 149 kDa
• Observed Molecular Weight: 145-150 kDa
• GenBank Accession Number: BC146764
• Gene Symbol: MYO6
• Gene ID (NCBI): 4646
• RRID: AB_2880631
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYO6, an actin-based motor protein, is the only myosin known to move toward the minus end of actin filaments. MYO6 is highly expressed in the inner and outer hair cells of the ear, retina, and polarized epithelial cells such as kidney proximal tubule cells and intestinal enterocytes. And it participates in a wide range of biological processes within cells, including clathrin-mediated endocytosis, vesicular membrane traffic, polarized secretion, and autophagy (PMID: 23620821; PMID: 28591580). Previous studies showed that MYO6 is upregulated in various types of cancer, and it has been widely reported to contribute to tumor cell migration and metastasis. Some articles indicate that MYO6 is associated with prostate cancer, lung cancer, human colorectal cancer and gastric cancer (PMID: 29022908).

Publications

Species	Application	Title
human	WB	Nat Commun; Structure of Myosin VI/Tom1 complex reveals a cargo recognition mode of Myosin VI for tethering.; Authors - Shichen Hu
human	WB,IHC	Cell Signal; Elevated expression of myosin VI contributes to breast cancer progression via MAPK/ERK signaling pathway; Authors - Xiao-Juan Zhan ; KD Validated
mouse	WB,IHC,IF	Nat Commun; MYL3 protects chondrocytes from senescence by inhibiting clathrin-mediated endocytosis and activating of Notch signaling; Authors - He Cao
mouse	IF	Int J Toxicol; Copper Chaperone Atox1 Protected the Cochlea From Cisplatin by Regulating the Copper Transport Family and Cell Cycle; Authors - Xubo Chen
human	WB	Int J Mol Sci; The Suppression of the Epithelial to Mesenchymal Transition in Prostate Cancer through the Targeting of MYO6 Using MiR-145-5p; Authors - Lee Armstrong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wojciech (Verified Customer) (04-20-2023)

We used this antibody to assess protein levels in human T cells. We got a good signal with 1:1000 dilution with observed molecular weight at ~150kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: T cells

FH

Sara (Verified Customer) (10-25-2020)

B cells (mouse) in RIPA buffer. 10 µg of protein per lane were loaded on a 10% PAGE-SDS gel. Transfer is not optimised for high MW proteins, so I could get a better blot. Two bands are detected, one of 150 kDa and one > 200 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse B cells from spleen

MYO6 Antibody Western Blot validation (1:1000 dilution) in Mouse B cells from spleen (Cat no:26778-1-AP)