MYO1F Polyclonal antibody

MYO1F Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28841-1-AP

Synonyms

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Validation Data Gallery

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  WB analysis using 28841-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28841-1-AP (MYO1F antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, rat spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28841-1-AP targets MYO1F in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYO1F fusion protein Ag29899
• Full Name: myosin IF
• Calculated Molecular Weight: 1098 aa, 125 kDa
• Observed Molecular Weight: 115-125 kDa
• GenBank Accession Number: BC028071
• Gene Symbol: MYO1F
• Gene ID (NCBI): 4542
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myosin family members are proteins that hydrolyze ATP to generate force or directional movement along actin filaments and are implicated in the rearrangement of the cytoskeleton in several cell types. Myosin 1F (MYO1F), a long tailed class I myosin, is highly expressed in natural killer (NK) cells, macrophages, dendritic cells, and neutrophils. MYO1F plays a critical role in the mediating of signaling molecules "trafficking from membrane to cytoplasm," and this process is essential for the antifungal signaling pathway activation.