MYLK3 Polyclonal antibody, PBS Only

MYLK3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 21527-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis using 21527-1-AP (same clone as 21527-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21527-1-AP (MYLK3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21527-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 21527-1-AP (same clone as 21527-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 21527-1-AP (MYLK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21527-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 21527-1-AP (same clone as 21527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 21527-1-AP (MYLK3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 21527-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 21527-1-AP (same clone as 21527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 21527-1-AP (MYLK3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 21527-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 21527-1-AP (same clone as 21527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 21527-1-AP (MYLK3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21527-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 21527-1-AP (same clone as 21527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 21527-1-AP (MYLK3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21527-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 21527-1-AP (same clone as 21527-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYLK3 antibody (21527-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21527-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 21527-1-AP (same clone as 21527-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 21527-1-AP (MYLK3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21527-1-PBS in a different storage buffer formulation.

---

Product Information

21527-1-PBS targets MYLK3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYLK3 fusion protein Ag16137
• Full Name: myosin light chain kinase 3
• Calculated Molecular Weight: 819 aa, 88 kDa
• Observed Molecular Weight: 85-100 kDa
• GenBank Accession Number: BC109097
• Gene Symbol: MYLK3
• Gene ID (NCBI): 91807
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Cardiac myosin light chain kinase (cMLCK) encoded by the myosin light chain kinase 3 (MYLK3) phosphorylates cardiac myosin light chain 2 (MLC2, encoded by MYL2) and facilitates actin-myosin interactions that enhance contractile force in the animal heart (PMID: 29235529).