MYL7 Polyclonal antibody

MYL7 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 17283-1-AP


MLC 2a, MLC2a, MYL2A, MYL7, MYLC2A, Myosin light chain 2a

Tested Applications

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse heart tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue
Positive FC detected inC2C12 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17283-1-AP targets MYL7 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYL7 fusion protein Ag11058
Full Name myosin, light chain 7, regulatory
Calculated Molecular Weight 175 aa, 19 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC027915
Gene Symbol MYL7
Gene ID (NCBI) 58498
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYL7, also known as myosin light chain 2a (MLC2a), is essential for heart development. The expression of MYL7 in heart is predominatantly restricted to the atrium. So its expression has been considered as useful marker for atrial cardiomyocytes.


Product Specific Protocols
WB protocol for MYL7 antibody 17283-1-APDownload protocol
IHC protocol for MYL7 antibody 17283-1-APDownload protocol
IF protocol for MYL7 antibody 17283-1-APDownload protocol
IP protocol for MYL7 antibody 17283-1-APDownload protocol
FC protocol for MYL7 antibody 17283-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Clin Invest

1-Deoxynojirimycin promotes cardiac function and rescues mitochondrial cristae in mitochondrial hypertrophic cardiomyopathy

Authors - Qianqian Zhuang

Nat Commun

High-efficiency reprogramming of fibroblasts into cardiomyocytes requires suppression of pro-fibrotic signalling.

Authors - Yuanbiao Zhao


Thymosin β4 increases cardiac cell proliferation, cell engraftment, and the reparative potency of human induced-pluripotent stem cell-derived cardiomyocytes in a porcine model of acute myocardial infarction.

Authors - Shi Hua Tan

Cardiovasc Res

Angiopoietin-1 enhanced myocyte mitosis, engraftment, and the reparability of hiPSC-CMs for treatment of myocardial infarction.

Authors - Zhonghao Tao

Stem Cell Reports

Mitochondrial Dysfunctions Contribute to Hypertrophic Cardiomyopathy in Patient iPSC-Derived Cardiomyocytes with MT-RNR2 Mutation.

Authors - Shishi Li

Front Cell Dev Biol

HiPS-Endothelial Cells Acquire Cardiac Endothelial Phenotype in Co-culture With hiPS-Cardiomyocytes.

Authors - Emmi Helle


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Brice-Emmanuel (Verified Customer) (12-18-2023)

Works very well in WB

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/5000
  • Cell Tissue Type: Mouse Heart Tissue
MYL7 Antibody Western Blot validation (1/5000 dilution) in Mouse Heart Tissue (Cat no:17283-1-AP)

Rebecca (Verified Customer) (10-25-2022)

20ug of protein were loaded. Transference was performed at 4ºC and 90V for 2h. Antibody incubation was done ON at 4ºC.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: iPSC-CM
MYL7 Antibody Western Blot validation (1:1000 dilution) in iPSC-CM (Cat no:17283-1-AP)