Myosin Light Chain 2/MLC-2V Polyclonal antibody

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  WB analysis of rat heart using 55462-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 55462-1-AP (MYL2 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 55462-1-AP

  IP result of anti-Myosin Light Chain 2/MLC-2V (IP:55462-1-AP, 4ug; Detection:55462-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human heart using 55462-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55462-1-AP (MYL2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 55462-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55462-1-AP (MYL2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 55462-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55462-1-AP (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 55462-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55462-1-AP (MYL2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 55462-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55462-1-AP (MYL2 Antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of C2C12 using 55462-1-AP

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human Myosin Light Chain 2/MLC-2V (55462-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Myosin Light Chain 2/MLC-2V Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Cat No. 55462-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC (Intra), IP, ELISA

Synonyms

MLC 2v, MYL2, MLC 2, MLC2, MLC-2

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Tested Applications

• Positive WB detected in: rat heart tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue, human heart tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:20000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

55462-1-AP targets Myosin Light Chain 2/MLC-2V in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: myosin, light chain 2, regulatory, cardiac, slow
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: NM_000432
• Gene Symbol: Myosin Light Chain 2
• Gene ID (NCBI): 4633
• RRID: AB_2881342
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P10916
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.

Publications

Species	Application	Title
mouse	IF	Stem Cell Reports; Non-genetic Purification of Ventricular Cardiomyocytes from Differentiating Embryonic Stem Cells through Molecular Beacons Targeting IRX-4.; Authors - Kiwon Ban
human	WB	iScience; SAA1/TLR2 axis directs chemotactic migration of hepatic stellate cells responding to injury.; Authors - Anteneh Getachew
human	WB	Int J Mol Med; Overexpression of CDCA8 promotes the malignant progression of cutaneous melanoma and leads to poor prognosis.; Authors - Chao Ci