MYH7B Polyclonal antibody

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  WB analysis using 22300-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22300-1-AP (MYH7B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

MYH7B Polyclonal Antibody for WB, ELISA

Cat No. 22300-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

Antigen MLAA 21, KIAA1512, MHC14, MYH14, MYH7B, Myosin 7B, Slow A MYH14

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22300-1-AP targets MYH7B in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: myosin, heavy chain 7B, cardiac muscle, beta
• Calculated Molecular Weight: 221 kDa
• Observed Molecular Weight: 200-230 kDa
• GenBank Accession Number: NM_020884
• Gene Symbol: MYH7B
• Gene ID (NCBI): 57644
• RRID: AB_3669394
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: A7E2Y1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myosin heavy chain 7b (MYH7b) is an ancient member of the myosin heavy chain motor protein family that is expressed in striated muscles. In mammalian cardiac muscle, MYH7b RNA is expressed along with two other myosin heavy chains, β-myosin heavy chain (β-MyHC) and α-myosin heavy chain (α-MyHC). However, unlike β-MyHC and α-MyHC, which are maintained in a careful balance at the protein level, the MYH7b locus does not produce a full-length protein in the heart due to a posttranscriptional exon-skipping mechanism that occurs in a tissue-specific manner (PMID: 33895132). MYH7b can be detected as about 200-230 kDa.