MYH4-Specific Polyclonal antibody

MYH4-Specific Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

34

Cat no : 20140-1-AP

Synonyms

MYH2B, MYH4, MYH4-Specific, MyHC 2B, MyHC IIb, Myosin 4, Myosin heavy chain 2b, Myosin heavy chain 4, Myosin heavy chain IIb

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Validation Data Gallery

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  WB analysis of human skeletal muscle using 20140-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20140-1-AP (MYH4-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 20140-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20140-1-AP (MYH4-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 20140-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20140-1-AP (MYH4-Specific Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 20140-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20140-1-AP (MYH4-Specific Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse skeletal muscle using 20140-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20140-1-AP (MYH4-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse skeletal muscle using 20140-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using MYH4-Specific antibody (20140-1-AP) at dilution of 1:200, MYH2-specific antibody (55069-1-AP, green), MYH7-specific antibody (22280-1-AP, blue).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, human skeletal muscle tissue, rat skeletal muscle tissue
• Positive IHC detected in: human skeletal muscle tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 28 publications below
• IHC: See 1 publications below
• IF: See 9 publications below

Product Information

20140-1-AP targets MYH4-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: myosin, heavy chain 4, skeletal muscle
• Calculated Molecular Weight: 223 kDa
• Observed Molecular Weight: 230 kDa
• GenBank Accession Number: NM_017533
• Gene Symbol: MYH4
• Gene ID (NCBI): 4622
• RRID: AB_10666434
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myosin is one of the most important skeletal muscle proteins. MYH4 functions muscle contraction. The antibody is specific to MYH4.

Publications

Species	Application	Title
rat	IHC,IF	Sci Adv; Bioactive fiber-reinforced hydrogel to tailor cell microenvironment for structural and functional regeneration of myotendinous junction; Authors - Yuzhi Sun
mouse	IF	Nat Commun; The nuclear envelope protein Net39 is essential for muscle nuclear integrity and chromatin organization.; Authors - Andres Ramirez-Martinez
mouse	IF	Development; The long noncoding RNA Meg3 regulates myoblast plasticity and muscle regeneration through epithelial-mesenchymal transition; Authors - Tiffany L. Dill
mouse	IF	Am J Pathol; Klotho null mutation involvement in adenosine A2B receptor-related skeletal muscle degeneration; Authors - Hung-Liang Pai
mouse	IF	Development; The long noncoding RNA Meg3 regulates myoblast plasticity and muscle regeneration through epithelial-mesenchymal transition.; Authors - Tiffany L Dill
mouse	WB	Int J Mol Sci; Loss of Myostatin Alters Mitochondrial Oxidative Phosphorylation, TCA Cycle Activity, and ATP Production in Skeletal Muscle; Authors - Xueqiao Wang

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