MYH2-specific Monoclonal antibody, PBS Only

• 

  WB analysis of human skeletal muscle using 66212-1-Ig (same clone as 66212-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66212-1-Ig (Myosin 2a antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66212-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 66212-1-Ig (same clone as 66212-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66212-1-Ig (Myosin 2a Antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 66212-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 66212-1-Ig (same clone as 66212-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66212-1-Ig (Myosin 2a Antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 66212-1-PBS in a different storage buffer formulation.

MYH2-specific Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66212-1-PBS

Clone No.1F1B6

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Myosin 2, MYH2, 1F1B6, MYHSA2, MyHC-IIa

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Product Information

66212-1-PBS targets MYH2-specific in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: myosin, heavy chain 2, skeletal muscle, adult
• Calculated Molecular Weight: 223 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: NM_017534
• Gene Symbol: MYH2
• Gene ID (NCBI): 4620
• RRID: AB_2881603
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UKX2
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MYH2 (Myosin II) is a member of the class II or conventional myosin heavy chains. Myosin II was first isolated from muscle but is also found in non muscle cells, and it is especially enriched in highly motile cell types such as amoebae. It forms bipolar filaments that interact with actin filaments to produce contraction. And it is the motor protein that generates force to drive muscle contraction. Functions in skeletal muscle contraction. The antibody is specific to MYH2, will not bind other myosins. It is a skeletal muscle specific antibody.