MYH14 Polyclonal antibody, PBS Only

MYH14 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20716-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 20716-1-AP (same clone as 20716-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20716-1-AP (MYH14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20716-1-PBS in a different storage buffer formulation.

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  WB analysis of human skeletal muscle using 20716-1-AP (same clone as 20716-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20716-1-AP (MYH14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20716-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse colon using 20716-1-AP (same clone as 20716-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 20716-1-AP (MYH14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20716-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 20716-1-AP (same clone as 20716-1-PBS)

  IP result of anti-MYH14 (IP:20716-1-AP, 4ug; Detection:20716-1-AP 1:2000) with mouse kidney tissue lysate 4800ug. This data was developed using the same antibody clone with 20716-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 20716-1-AP (same clone as 20716-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20716-1-AP (MYH14 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20716-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 20716-1-AP (same clone as 20716-1-PBS)

  Immunofluorescent analysis of Hela cells, using MYH14 antibody 20716-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 20716-1-PBS in a different storage buffer formulation.

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Product Information

20716-1-PBS targets MYH14 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: myosin, heavy chain 14
• Calculated Molecular Weight: 228 kDa
• Observed Molecular Weight: 228 kDa
• GenBank Accession Number: NM_024729
• Gene Symbol: MYH14
• Gene ID (NCBI): 79784
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MYH14, also named as KIAA2034 and NMHC II-C, is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Defects in MYH14 are the cause of deafness autosomal dominant type 4 (DFNA4). The antibody is specific to MYH14.