Validation Data Gallery
|Positive WB detected in
|mouse uterus tissue
|Positive IHC detected in
|human colon tissue, rat eye tissue, mouse eye tissue, rat bladder tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|mouse heart tissue
|Positive FC detected in
|Western Blot (WB)
|WB : 1:1000-1:4000
|IHC : 1:1000-1:4000
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
21404-1-AP targets SMMHC in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|SMMHC fusion protein Ag16114
|myosin, heavy chain 11, smooth muscle
|Calculated molecular weight
|1979 aa, 228 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
SMMHC (smooth muscle myosin heavy chain; MYH11) is a contractile protein of smooth muscle cells. It is specifically expressed in cells derived from smooth muscle lineages. SMMHC is used as vascular smooth muscle cell (vSMC) contractile marker. It is also an excellent marker for myoepithelial cells, with no or few cross-reaction with myofibroblasts, thus very useful in the evaluation of stromal invasion.
|Product Specific Protocols
|WB protocol for SMMHC antibody 21404-1-AP
|IHC protocol for SMMHC antibody 21404-1-AP
|IF protocol for SMMHC antibody 21404-1-AP
|FC protocol for SMMHC antibody 21404-1-AP
|Click here to view our Standard Protocols
Regulated lysosomal exocytosis mediates cancer progression.
J Clin Invest
UHRF1 epigenetically orchestrates smooth muscle cell plasticity in arterial disease.
Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.
Acta Physiol (Oxf)
Prostacyclin facilitates vascular smooth muscle cell phenotypic transformation via activating TP receptors when IP receptors are deficient.
Nesfatin-1 promotes VSMC migration and neointimal hyperplasia by upregulating matrix metalloproteinases and downregulating PPARγ.
Oxid Med Cell Longev
S-Nitroso-L-Cysteine Ameliorated Pulmonary Hypertension in the MCT-Induced Rats through Anti-ROS and Anti-Inflammatory Pathways.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tom (Verified Customer) (07-12-2023)
One of the best smooth muscle cell markers for immunofluorescence!!