SMMHC Polyclonal antibody

SMMHC Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 21404-1-AP

Synonyms

MYH11, Myosin 11, Myosin heavy chain 11, SMHC, SMMHC, SMM-HC, smooth muscle myosin heavy chain



Tested Applications

Positive WB detected inmouse uterus tissue
Positive IHC detected inhuman colon tissue, rat eye tissue, mouse eye tissue, rat bladder tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue
Positive FC detected inC2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

21404-1-AP targets SMMHC in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMMHC fusion protein Ag16114
Full Name myosin, heavy chain 11, smooth muscle
Calculated molecular weight 1979 aa, 228 kDa
Observed molecular weight 200 kDa
GenBank accession numberBC104906
Gene symbol MYH11
Gene ID (NCBI) 4629
RRIDAB_10732819
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMMHC (smooth muscle myosin heavy chain; MYH11) is a contractile protein of smooth muscle cells. It is specifically expressed in cells derived from smooth muscle lineages. SMMHC is used as vascular smooth muscle cell (vSMC) contractile marker. It is also an excellent marker for myoepithelial cells, with no or few cross-reaction with myofibroblasts, thus very useful in the evaluation of stromal invasion.

Protocols

Product Specific Protocols
WB protocol for SMMHC antibody 21404-1-APDownload protocol
IHC protocol for SMMHC antibody 21404-1-APDownload protocol
IF protocol for SMMHC antibody 21404-1-APDownload protocol
FC protocol for SMMHC antibody 21404-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Sci Adv

Regulated lysosomal exocytosis mediates cancer progression.

Authors - Eda Machado
humanWB

J Clin Invest

UHRF1 epigenetically orchestrates smooth muscle cell plasticity in arterial disease.

Authors - Leonardo Elia
mouseWB

Theranostics

Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.

Authors - Gao-Shan Yang
ratWB

Acta Physiol (Oxf)

Prostacyclin facilitates vascular smooth muscle cell phenotypic transformation via activating TP receptors when IP receptors are deficient.

Authors - Ziqing Li
ratWB

Biomed Pharmacother

Nesfatin-1 promotes VSMC migration and neointimal hyperplasia by upregulating matrix metalloproteinases and downregulating PPARγ.

Authors - Ji-Ru Zhang
ratWB

Oxid Med Cell Longev

S-Nitroso-L-Cysteine Ameliorated Pulmonary Hypertension in the MCT-Induced Rats through Anti-ROS and Anti-Inflammatory Pathways.

Authors - Moran Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tom (Verified Customer) (07-12-2023)

One of the best smooth muscle cell markers for immunofluorescence!!

  • Applications: Immunofluorescence