MYH10 Polyclonal antibody

MYH10 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 21403-1-AP

Synonyms

Myosin 10, NMMHC-B, NMMHC II-b, Myosin-10, Myosin heavy chain, non-muscle IIb

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Validation Data Gallery

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  WB analysis using 21403-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21403-1-AP (MYH10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 21403-1-AP

  IP result of anti-MYH10 (IP:21403-1-AP, 4ug; Detection:21403-1-AP 1:1500) with mouse brain tissue lysate 3440ug.

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  IF Staining of HepG2 using 21403-1-AP

  Immunofluorescent analysis of HepG2 cells, using MYH10 antibody 21403-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain
• Positive IP detected in: mouse brain tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Product Information

21403-1-AP targets MYH10 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYH10 fusion protein Ag16113
• Full Name: myosin, heavy chain 10, non-muscle
• Calculated Molecular Weight: 1985 aa, 230 kDa
• Observed Molecular Weight: 229 kDa
• GenBank Accession Number: BC150634
• Gene Symbol: MYH10
• Gene ID (NCBI): 4628
• RRID: AB_10804351
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYH10 (non-muscle II-b, NM IIB) is a member of non-muscle myosin II which plays fundamental roles in the maintenance of cell morphology, cell adhesion, and migration, as well as cell division. MYH10 is mainly present in nerve cells, megakaryocytes, and other non-muscle cells. It has been reported to mediate centrosome reorientation during cell migration and contribute to ciliogenesis. Overexpression of MYH10 has been observed in breast cancer.

Publications

Species	Application	Title
bovine	WB,IP,IF	J Agric Food Chem; LncRNA BlncAD1 Modulates Bovine Adipogenesis by Binding to MYH10, PI3K/Akt Signaling Pathway, and miR-27a-5p/CDK6 Axis; Authors - Xin Li
human	WB,IP,IF	Nature; TMEFF1 is a neuron-specific restriction factor for herpes simplex virus; Authors - Yao Dai