MYH10 Polyclonal antibody

MYH10 Polyclonal Antibody for FC, IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 21403-1-AP

Synonyms

MYH10, Myosin 10, Myosin heavy chain 10

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Validation Data Gallery

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  WB analysis using 21403-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21403-1-AP (MYH10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 21403-1-AP

  IP result of anti-MYH10 (IP:21403-1-AP, 4ug; Detection:21403-1-AP 1:1500) with mouse brain tissue lysate 3440ug.

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  IF Staining of HepG2 using 21403-1-AP

  Immunofluorescent analysis of HepG2 cells, using MYH10 antibody 21403-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HepG2 using 21403-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human MYH10 (21403-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain
• Positive IP detected in: mouse brain tissue
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21403-1-AP is MYH10 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYH10 fusion protein Ag16113
• Full Name: myosin, heavy chain 10, non-muscle
• Calculated Molecular Weight: 1985 aa, 230 kDa
• Observed Molecular Weight: 229 kDa
• GenBank Accession Number: BC150634
• Gene Symbol: MYH10
• Gene ID (NCBI): 4628
• RRID: AB_10804351
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYH10 (non-muscle II-b, NM IIB) is a member of non-muscle myosin II which plays fundamental roles in the maintenance of cell morphology, cell adhesion, and migration, as well as cell division. MYH10 is mainly present in nerve cells, megakaryocytes, and other non-muscle cells. It has been reported to mediate centrosome reorientation during cell migration and contribute to ciliogenesis. Overexpression of MYH10 has been observed in breast cancer.