MYH1-motor domain Polyclonal antibody, PBS Only

MYH1-motor domain Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 22282-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of human skeletal muscle using 22282-1-AP (same clone as 22282-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22282-1-AP (MYH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22282-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 22282-1-AP (same clone as 22282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22282-1-AP (MYH1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22282-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 22282-1-AP (same clone as 22282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22282-1-AP (MYH1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22282-1-PBS in a different storage buffer formulation.

• 

  FC experiment of C2C12 using 22282-1-AP (same clone as 22282-1-PBS)

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human MYH1-motor domain (22282-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 22282-1-PBS in a different storage buffer formulation.

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Product Information

22282-1-PBS targets MYH1-motor domain in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: myosin, heavy chain 1, skeletal muscle, adult
• Calculated Molecular Weight: 223 kDa
• Observed Molecular Weight: 223 kDa
• GenBank Accession Number: NM_005963
• Gene Symbol: MYH1
• Gene ID (NCBI): 4619
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.