MYH1 (N-terminal) Polyclonal antibody

MYH1 (N-terminal) Polyclonal Antibody for IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 25182-1-AP

Synonyms

MYH1, Myosin-1, Myosin 1, MyHC-IIx/d, MyHC-2x

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Validation Data Gallery

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  IP experiment of mouse skeletal muscle using 25182-1-AP

  IP result of anti-MYH1 (N-terminal) (IP:25182-1-AP, 5ug; Detection:25182-1-AP 1:1000) with mouse skeletal muscle tissue lysate 4000ug.

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  IHC staining of human skeletal muscle using 25182-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25182-1-AP (MYH1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 25182-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25182-1-AP (MYH1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse skeletal muscle using 25182-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using MYH1 (N-terminal) antibody (25182-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse skeletal muscle tissue

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

25182-1-AP targets MYH1 (N-terminal) in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYH1 (N-terminal) fusion protein Ag17129
• Full Name: myosin, heavy chain 1, skeletal muscle, adult
• Calculated Molecular Weight: 1939 aa, 223 kDa
• Observed Molecular Weight: 220-230 kDa
• GenBank Accession Number: BC114545
• Gene Symbol: MYH1
• Gene ID (NCBI): 4619
• RRID: AB_2879947
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.

Publications

Species	Application	Title
mouse	WB	Int J Biol Macromol; Eicosapentaenoic acid-mediated activation of PGAM2 regulates skeletal muscle growth and development via the PI3K/AKT pathway; Authors - Chenchen Li
	WB,IF	Int J Mol Sci; miRNA-10a-5p Targeting the BCL6 Gene Regulates Proliferation, Differentiation and Apoptosis of Chicken Myoblasts; Authors - Genxi Zhang
mouse	WB	J Agric Food Chem; Spermidine-Activated Satellite Cells Are Associated with Hypoacetylation in ACVR2B and Smad3 Binding to Myogenic Genes in Mice.; Authors - Luchu Zhang
	WB,IHC	Cells; MicroRNA-27b-3p Targets the Myostatin Gene to Regulate Myoblast Proliferation and Is Involved in Myoblast Differentiation.; Authors - Genxi Zhang
mouse	WB	J Agric Food Chem; Naringin Promotes Skeletal Muscle Fiber Remodeling by the AdipoR1-APPL1-AMPK Signaling Pathway.; Authors - Peiyuan Li
human,mouse	WB	Sci Rep; MicroRNA-494-3p inhibits formation of fast oxidative muscle fibres by targeting E1A-binding protein p300 in human-induced pluripotent stem cells.; Authors - Hirotaka Iwasaki