MYD88 Polyclonal antibody

MYD88 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 29946-1-AP

Synonyms

Myd88

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Validation Data Gallery

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  WB analysis using 29946-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29946-1-AP (MYD88 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 29946-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29946-1-AP (MYD88 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 29946-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29946-1-AP (MYD88 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: NR8383 cells, RAW 264.7 cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

29946-1-AP targets MYD88 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYD88 fusion protein Ag30662
• Full Name: myeloid differentiation primary response gene 88
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 29-34 kDa
• GenBank Accession Number: NM_010851
• Gene Symbol: Myd88
• Gene ID (NCBI): 17874
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYD88 is a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. MYD88 is the key adaptor protein for the interleukin-1 receptor (IL-1R) and most Toll-like receptors (TLRs), which are essential for innate immunity and pathogen-associated molecular pattern recognition. Mutations in the MyD88 gene lead to the development of cancer in humans and mice suggesting that MyD88 also plays a cell autonomous role in tissue homeostasis. MYD88 is detected in bone marrow.

Publications

Species	Application	Title
rat	WB	Int Immunopharmacol; Echinatin alleviates inflammation and pyroptosis in hypoxic-ischemic brain damage by inhibiting TLR4/ NF-κB pathway; Authors - Xiaoyue Tao